Protective efficacy of H antigen from Histoplasma capsulatum in a murine model of pulmonary histoplasmosis

We previously reported that immunization with H antigen from Histoplasma ca psulatum did not protect mice against an intravenous challenge with yeasts, Here, we investigated the utility of H antigen to protect mice in a model of pulmonary histoplasmosis. Mice immunized with H antigen and challenged i ntranasally 4 weeks postvaccination were protected against sublethal and le thal challenges with H, capsulatum yeasts. If the challenge was performed 3 months after vaccination, there was a reduction in fungal burden following sublethal challenge and a modest delay in mortality in mice given a lethal inoculum, Vaccination was associated with production of gamma interferon, granulocyte-macrophage colony-stimulating factor, interleukin-4, and interl eukin-10 by splenocytes. Vaccination with H antigen was not accompanied by a major expansion of CD4(+) or CD8(+) cells in spleens of mice. These resul ts demonstrate that Il antigen may be useful as a protective immunogen agai nst pulmonary exposure to H. capsulatum..