A combination analysis of p53 and p21 in gastric carcinoma as a strong indicator for prognosis

We studied p53 and p21 expression simultaneously in gastric carcinoma tissu es to investigate the clinical significance of p53-p21 pathway in this dise ase. One hundred sixty-four primary gastric carcinoma specimens were immuno histochemically stained for p53 and p21 protein, and clinicopathological fe atures of the cases were examined. P53 was stained negatively, while p21 wa s stained positively in each normal stomach epithelium. P53, and p21 positi ve staining was observed in 82 (50%) and 61 (37.2%) tumors, respectively. U nexpectingly, no correlation was found between p53 and p21 staining status. Tumors demonstrating preserved p53-p21 pathway [p53(-)/p21(+)], observed i n 20.1% of the tumors, displayed less aggressive characteristics, and no re current disease after curative resection. While tumors demonstrating disrup ted p53-p21 pathway [p53(+)/p21(-)], observed in 32.9% of the tumors, displ ayed significantly more aggressive characteristics, poorer survival and hig her recurrence rate than the tumors demonstrating other staining patterns. P53-p21 pathway was widely altered in gastric carcinomas. The combined eval uation of p53 and p21 expression in gastric carcinoma tissues is suggested to have clinical importance by indicating not only the malignant potential of each tumor, but also the prognosis of this disease.