p53-binding consensus-like sequence (T3SF) is located in the murine promote
r region of tissue inhibitor of metalloproteinase 3 gene. To identify the g
enes that encode proteins that bind to T3SF DNA sequence, we screened a cDN
A library using the Southwestern technique. The SMRT gene was cloned as one
of the candidates. Addition of antibody against SMRT reduced the intensity
of a band that is supposed to contain SMRT in electrophoresis mobility shi
ft assay, although antibody against p53 had no effect. Ultraviolet (UV)-irr
adiation reduced the intensity of the SMRT complex whereas p53 complex was
stabilized by UV-irradiation. These results suggest that SMRT may bind to T
3SF sequence in p53-independent manner and dissociate from the sequence by
UV irradiation.