CrossLaps and beta-glucuronidase in peri-implant and gingival crevicular fluid

Collagen degradation products of the carboxyterminal region possibly reflec t bone and attachment loss. In the present study, the Serum CrossLaps One-S tep enzyme-linked immunosorbent assay was used to determine a specific part of the carboxyterminal region of type I collagen, the CrossLaps. Samples o f peri-implant and gingival crevicular fluid of 111 implants and 53 teeth f rom 47 partially or completely edentulous patients were examined in referen ce to levels of CrossLaps and beta -glucuronidase (betaG), an established m arker of periodontal disease. Clinical probing pocket depth (PPD), bleeding on probing (BOP), plaque accumulation, mobility, radiographic bone loss, a nd the occurrence of Actinobacillus actinomycetemcomitans, Porphyromonas gi ngivalis, and Prevotella intermedia were assessed. The mean values were: fo r PPD at implants 3.76 +/- 1.41 mm, at teeth 3.44 +/- 0.88 mm; for betaG at implants 0.364 +/- 0.392 pU/min, at teeth 0.314 +/- 0.209 pU/min; for Cros sLaps at implants 0.069 +/- 0.059 pmol/min, at teeth 0.082 +/- 0.053 pmol/m in. Bleeding on probing was significantly higher on implants than on teeth (McNemar test, P = .004). No significant difference of betaG levels was fou nd between teeth and implants (Wilcoxon test). A negative correlation was f ound between betaG levels and CrossLaps levels at teeth (Pearson-rank corre lation, P = .002). On implants, no significant correlation of these 2 param eters was seen, but significant correlations were found between sulcus flui d flow rate and PPD (P = .012), betaG levels and bone loss (P < 0.0005), an d CrossLaps levels and PPD (P = .011). CrossLaps can be detected in both gi ngival and peri-implant crevicular fluid. While rising levels of <beta>G ma y indicate acute peri-implantitis, CrossLaps may not, but could play a role as a marker of ongoing attachment loss.