Adsorption of urokinase by polypropylene films with various amine groups

Polypropylene films with various amine groups were prepared by radiation-in duced grafting of glycidyl methacrylate (GMA) onto polypropylene (PP) films and the subsequent amination of poly-GMA graft chains were examined. The p hysical and chemical properties of the GMA-grafted PP film and the aminated PP film were investigated by IR, SEM, XPS, and TGA. The adsorption of urok inase for the PP films modified with various amine groups was examined unde r various conditions of different amine group contents and pH values. The a dsorption of urokinase increased by increasing the amine group content. In the range of amine group content from 1.0 to 2.7 mmol/g, the adsorption of urokinase by the PP films modified with six kinds of amines was in the foll owing order: trimethylamine > diethylenetriamine > triethylenetetraamine > ethylenediamine > dimethylamine > diethyleneamine. The adsorption amounts o f urokinase by the PP film with the trimethylamine and triethylenetetraamin e groups at pH 7.4 were higher than those at pH 9. (C) 2001 John Wiley & So ns, Inc.