Hydrophobic interaction chromatography of proteins

In this article. an overview of hydrophobic interaction chromatography (HIC ) of proteins is given. After a brief description of protein hydrophobicity and hydrophobic interactions, we present the different proposed theories f or the retention mechanism of proteins in HIC. Additionally, the main param eters to consider for the optimization of fractionation processes by HIC an d the stationary phases available were described. Selected examples of prot ein fractionation by HIC are also presented. (C) 2001 Elsevier Science B.V. All rights reserved.