Selectivity of amino-, cyano- and diol-bonded silica in reversed-phase liquid chromatography

Amino-, cyano- and diol-bonded silica stationary phases were characterized by estimating their characteristic interaction constants in reversed-phase liquid chromatography (RPLC) based on linear solvation energy relationships . Five characteristic interaction constants of the stationary phases, the h ydrophobicity (v), polarizability (r), dipolarity (s), hydrogen bond (HB) a cceptor basicity (a) and HE donor acidity strength (b) were determined by m ultiple regression analyses of logarithmic retention factors (k) for a set of test solutes measured on them in 10% (v/v) methanol-water vs. the solute properties represented by characteristic molecular volume (V-x), excess po larization (R-2), dipolarity/polarizability (pi*), HE donor acidity (alpha) and HE acceptor basicity (beta). Magnitudes of the five constants for the phases in RPLC were compared with those in normal-phase LC to see the diffe rences in chromatographic selectivity in the two LC modes. (C) 2001 Elsevie r Science B.V. All rights reserved.