Liquid chromatography of troleandomycin

Until now no liquid chromatography (LC) method is described to determine th e purity and content of troleandomycin and its related substances. A simple , robust, sensitive and selective isocratic liquid chromatographic method s uitable for the determination of the antibiotic troleandomycin and its rela ted substances is described. This method utilizes as a stationary phase: XT erra RP18 5 mum (25 cmX4.6 mm I.D.) at 30 degreesC and as mobile phase: ace tonitrile-0.2 M ammonium acetate buffer (pH 6.0)-water (45:5:50, v/v), deli vered at a flow-rate of 1.0 ml/min. W detection is performed at 205 nm. Tro leandomycin is separated from the partially acetylated related substances a nd from several unknown impurities present in commercial samples. The robus tness of the method was evaluated by a full-factorial experimental design. (C) 2001 Elsevier Science B.V. All rights reserved.