Repeatability and reproducibility of retention data and band profiles on reversed-phase liquid chromatography columns V. Results obtained with Vydac 218TP C-18 columns

The general goal of this work is to investigate the precision of chromatogr aphic data and to determine which properties of chromatographic columns inf luence this factor. Chromatographic data were acquired under five different sets of experimental conditions for 30 neutral, acidic and basic test comp ounds on columns packed with Vydac 218TP C-18, a polymeric, wide-pore silic a-based stationary phase. Five columns packed with samples from the same ba tch of this packing material were used to measure the column-to-column repr oducibility and six columns packed with material from six different batches to measure the batch-to-batch reproducibility. The parameters studied were the retention time, the retention and separation factors, the hydrophobic and the steric selectivities, the column efficiency, and the tailing factor . (C) 2001 Elsevier Science B.V. All rights reserved.