Sm. Fields et al., Superheated water as eluent in high-temperature high-performance liquid chromatographic separations of steroids on a polymer-coated zirconia column, J CHROMAT A, 913(1-2), 2001, pp. 197-204
High-temperature liquid chromatography (HTLC),;with a superheated water mob
ile phase, has been shown to be a feasible replacement for medium-polarity
acetonitrile-water mixtures as an eluent in reversed-phase HPLC, instrument
al parameters of flow-rate, injection volume and mobile phase preheating we
re shown to have significant effects on the quality of the chromatographic
peaks. The selectivity and retention patterns of testosterone and several r
elated compounds were investigated on a porous zirconia, polybutadiene-coat
ed column at temperatures up to 200 degreesC and compared with that of a po
rous silica, octadecylsilane-coated column and the zirconia column under tr
aditional reversed-phase conditions of an acetonitrile-water mobile phase a
t 40 degreesC. The selectivity differences observed for testosterone and re
lated compounds show that the separation mechanisms are complementary and u
nique selectivity is obtained with the zirconia column under HTLC condition
s. (C) 2001 Elsevier Science B.V. All rights reserved.