Application of high-performance liquid chromatography with isotope-ratio mass spectrometry for measuring low levels of enrichment of underivatized materials
Fp. Abramson et al., Application of high-performance liquid chromatography with isotope-ratio mass spectrometry for measuring low levels of enrichment of underivatized materials, J CHROMAT A, 913(1-2), 2001, pp. 269-273
Combining HPLC separations with an isotope-ratio mass spectrometric (IRMS)
detection produces a device capable of measuring very low alterations in C-
13 abundance from analyte species that cannot be volatilized. Examples are
presented showing proteins, carbohydrates, and nucleotides that are eluted
from varying types of HPLC columns (reversed-phase, normal-phase, ion-excha
nge and size-exclusion). This wide range of chromatographic methods enables
the analysis of compounds never before amenable to IRMS techniques and may
lead to the development of many new assays. (C) 2001 Elsevier Science BN.
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