Bupropion hydrochloride: the development of a chiral separation using an ovomucoid column

The separation of bupropion enantiomers on an ovomucoid stationary phase wa s investigated. The mobile- and stationary-phase parameters that may influe nce the separation were identified. The parameters that were studied includ e: type and concentration of organic modifier, mobile phase pH, ionic stren gth, type of buffer, and column temperature, as well as the effect that the amount of sample injected had on the separation. The optimized chiral sepa ration baseline-resolved the enantiomers in less than 10 min. Calibration c urves for a standard were linear over a range of 0.27-53.0 mug/g (ppm) with a correlation coefficient of 0.999 for bath enantiomers. A detection limit of 0.13 mug/g and a quantitation limit of 0.27 mug/g wen also found. The s ystem precision of the method was 0.2%. (C) 2001 Elsevier Science B.V. All rights reserved.