A functional S-allele, 'Sg', in the wild apple possessing a single amino acid, S-RNase 'Sg'-RNase', different from 'Sg-RNase' in Malus x domestica cultivars
S. Matsumoto et al., A functional S-allele, 'Sg', in the wild apple possessing a single amino acid, S-RNase 'Sg'-RNase', different from 'Sg-RNase' in Malus x domestica cultivars, J HORT SCI, 76(2), 2001, pp. 163-166
S3-, S5-, S9- (=Sc-), Sf-, Sg- and Sh (=S24)-alleles in the wild apple were
deduced by using S-allele-specific-polymerase-chain reaction (PCR)-restric
tion fragment length polymorphism (RFLP) analyses. Within the alleles, the
deduced amino acid sequence of Sg'-RNase in Sg-allele of Malus transitoria
differed by one amino acid from that of Sg-RNase in Sg-allele of M. x domes
tica 'Indo'. From the cross pollination analysis of M. transitoria and M. x
domestica 'Indo', and S-allele-specific-PCR-RFLP analyses of their progeni
es, it was indicated that the pistil Sg'-RNase in M. transitoria rejected t
he pollen of Sg-allele from 'Indo'. A difference of one amino acid within t
he RHV region produced by natural mutation was not enough to generate a new
S-allele. This is the first report in the literature concerning S-allele d
iversification in the apple.