Coordination of heavy metals by dithiothreitol, a commonly used thiol group protectant

Citation
A. Krezel et al., Coordination of heavy metals by dithiothreitol, a commonly used thiol group protectant, J INORG BIO, 84(1-2), 2001, pp. 77-88
Citations number
48
Categorie Soggetti
Biochemistry & Biophysics","Inorganic & Nuclear Chemistry
Journal title
JOURNAL OF INORGANIC BIOCHEMISTRY
ISSN journal
01620134 → ACNP
Volume
84
Issue
1-2
Year of publication
2001
Pages
77 - 88
Database
ISI
SICI code
0162-0134(200103)84:1-2<77:COHMBD>2.0.ZU;2-0
Abstract
D,L-Dithiothreitol (DTT), known also as Cleland reagent, is a thiol group p rotectant, used commonly in peptide and protein chemistry. Therefore, it is often added at high concentrations in preparations of proteins relevant to heavy metal biochemistry. The coordination of five of these metal ions, Zn (II), Cd(II), Pb(II), NI(II) and Cu(I) to DTT was studied by means of poten tiometric titrations, and UV-Vis and NMR spectroscopies. It was found that DTT forms specific and very stable polymeric and monomeric complexes with a ll of these metal ions, using both of its sulfur donors. The quantitative d escription of these complexes in solution and the solid state provides the basis for predictions of interference from DTT in studies of metal ion bind ing of thiol-containing biomolecules. (C) 2001 Elsevier Science B.V. All ri ghts reserved.