A new cyclic peptide 1 having an (LLLD)(3) configuration pattern was design
ed that is capable of forming artificial transmembrane ion channels by self
-assembly of planar peptide rings, with hydrophilic groups arrayed in the i
nterior of the channel. ion permeability in the presence of the synthetic p
eptide 1, cyclo[-Trp-Dap-Leu-D-Ala-Trp-Ser-Val-D-Ala-Trp-Ser-Ile-Gly-] (Dap
: L-diaminopropionic acid), was observed in lipid bilayer membranes. The pH
dependence of ionic conductance showed that the beta -amino group of Dap m
ay play a role in the conductance of the peptide channels. Fourier-transfor
m infrared and circular dichroism data imply that, in a membrane, a stack o
f cyclic peptides is formed in which the inter peptide H bonds form a kind
of beta -structure analogous to that in the gramicidin A dimer and distinct
from the H-bonding pattern of the beta -barrels.