Determination of Norfloxacin by square-wave adsorptive voltammetry on a glassy carbon electrode

The adsorptive and electrochemical behavior of norfloxacin on a glassy carb on electrode were investigated by cyclic and square-wave voltammetry. Cycli c voltammetric studies indicated that the process was irreversible and fund amentally controlled by adsorption. To obtain a good sensitivity. the solut ion conditions and instrumental parameters were studied using square-wave v oltammetry. In acetate buffer of pH 5.0. nurfloxacin gave a sensitive adsor ptive oxidative peak at 0.920 V (versus Ag-AgCl). Applicability to measurem ent of norfloxacin at submicromolar levels in urine samples was illustrated . The peak current was linear with the norfloxacin concentration in the ran ge 5-50 mug mi urine. The detection limit was 1.1 mug ml(-1) urine. (C) 200 1 Elsevier Science B.V. All rights reserved.