FIA of sildenafil citrate using UV-detection

how injection analysis (FIA) of sildenafil citrate (SLD) using UV detection is described in this study. The best solvent system was found to be consis ting of 0.2 M phosphate buffer at pH 8 having 10% MeOH. A flow rate of 1 ml . min(-1) was pumped and active material was detected at 292 nm. The calibr ation equation was linear in the range of 1 x 10(-6)-5 x 10 (-6) M. Limit o f detection (LOD) and limit of quantitation (LOQ) were calculated to be 3 x 10(-7) and 8.9 x 10(-7) M with a R.S.D. 1.9 and 0.6%, (n = 7), respectivel y. The proposed method was applied to the determination of SLD in VIAGRA (R ); tablet, containing 50 mg active material. The results were compared with those obtained from UV-Spectrophotometry. The results showed that there is a good agreement between FIA method and the UV-Spectrophotomrtry. The vali dation studies were realised by the related applications and the results we re evaluated statistically. According to the results. insignificant differe nce was observed between the methods. (C) 2001 Elsevier Science B.V. All ri ghts reserved.