LC method for the analysis of acetylsalicylic acid, caffeine and codeine phosphate in pharmaceutical preparations

An accurate, simple, reproducible and sensitive method for the determinatio n of acetylsalicylic acid, caffeine and codeine phosphate has been develope d and validated. Acetylsalicylic acid, caffeine and codeine phosphate were separated using a mu Bofidapack C-8 column by isocratic elution with flow r ate 1.0 ml/min. The mobile phase composition was 125/125/250/0.5 (v/v) isop ropyl alcohol, acetonitrile. water and omicron -phosphoric acid. The sample s were detected at 215 nm using photo-diode array detector. The linear rang e of detection for acetylsalicylic acid, caffeine and codeine phosphate wer e between 0.40 and 1000, 0.25 and 250, and 0.48 and 96 mug/ml, respectively . The linearity, range, selectivity, system performance parameters, precisi on, accuracy, and ruggedness for acetylsalicylic acid, caffeine and codeine phosphate were also shown to have acceptable values. (C) 2001 Elsevier Sci ence B.V. All rights reserved.