Sensory profiling of Scotch whiskies, important for research and product an
alysis, requires training of assessors including the identification of impo
rtant whisky aroma characters, Seventeen compounds were selected as potenti
al reference standards for common whisky sensory characteristics, Nineteen
assessors determined detection and recognition thresholds for each referenc
e standard dissolved in 3 year old grain whisky at 23 % (v/v) ethanol using
both ascending dilution series and Latin square presentations. With the fo
rmer order effects and errors of habituation and anticipation were apparent
. However, the Latin square presentation was satisfactory for threshold mea
surements in whisky, yielding good correlations and reproducibility, and en
abling outliers to be reassessed independently. Slopes obtained from plotti
ng probit against concentration for detection and recognition data, psychom
etric functions of aroma perception, varied between reference compounds and
90% recognition concentrations were obtained for each aroma standard. Sixt
een of the compounds assessed were suitable for use as reference flavour st
andards for training of whisky assessors.