Chromosomal positioning of human T-lymphotropic type 1 proviruses by fluorescence in situ hybridisation

Fluorescence in situ hybridisation (FISH) was employed to identify the chro mosomal integration site of the human T-cell lymphotropic virus, type 1 (HT LV-1) present in T-cell clones derived from HTLV-l-infected individuals and a virally transformed cell line, C8166-35. Proviral sequences were detecte d in C8166-45 but not uninfected Jurkat cells. Integration sites were relia bly detected in T-cell clones determined previously to be infected with HTL V-1. The results indicated that the transformed cell line and some of the T -cell clones possessed more than one proviral integration site. This hybrid isation system is: useful for determining the number of integration events and for localising proviruses to specific chromosomal regions. (C) 2001 Els evier Science B.V. All rights reserved.