Purification of woodchuck hepatitis surface antigen using a monoclonal antibody raised against the antigen

Woodchuck hepatitis virus (WHV) is closely related to the human hepatitis B virus (HBV) and infection of woodchucks with WHV creates a useful model fo r studies of immunity, pathogenesis and therapy of HBV infection. To increa se the usefulness of this model, monoclonal antibodies were raised to woodc huck hepatitis surface antigen (WHsAg) and one of these antibodies was used to purify the antigen by affinity chromatography from serum, a simpler and quicker method of purification than the current ultracentrifugation method s. The bands found by SDS-polyacrylamide gel electrophoresis of WHsAg were the major 25 and 29 kilodalton (kDa) bands and a triplet of 45, 51 and 55 k Da which are thought to be the glycosylated and unglycosylated middle and l arge WHsAg. Both the antibody and the antigen are valuable reagents for the study of WHV infection. (C) 2001 Elsevier Science B.V. All rights reserved .