Analysis of oxygenated cholesterol derivatives by planar chromatography

Oxygenated cholesterol derivatives have been separated on silica gel and si lanized silica gel TLC plates in an SDII horizontal chamber. Spots were vis ualized under UV light after reaction with different specific reagents for cholesterol and its derivatives. Cholesterol derivatives were also silanize d and analyzed by planar chromatography and the results were compared with those obtained by GC-MS. Cholesterol derivatives were also quantified by de nsitometry after isolation of the cholesterol derivatives fraction from ser um samples by solid-phase extraction.