Formation of self-assembled, air-stable lipid bilayer membranes on solid supports

A successful strategy for the self-assembly and stabilization of a substrat e-supported, phospholipid bilayer is described. The bilayer is self-organiz ed by fusion of fluid vesicles, composed of bis-sorbylphosphatidylcholine, on an oxide surface. The supported bilayer is then polymerized in situ to p roduce a cross-linked structure that is stable to surfactant solutions, org anic solvents, and to transfer across the air/water interface, yet retains the resistance to nonspecific protein adsorption characteristic of a fluid phosphatidylcholine bilayer.