Certain factors affecting the production of cell-associated alpha-gala
ctosidase by Bacillus sp. JF(2) were investigated. The intention was t
o maximize alpha-galactosidase activity of potential commercial applic
ation, by consecutive optimization of growth media and conditions. The
highest alpha-galactosidase activity was obtained when grown on melib
iose, whereas sucrose inhibited the production of alpha-galactosidase.
alpha-Galactosidase production was optimally active at pH 7.5 and 55
degrees C. It was identified that a soy effluent stream could be used
as the best carbon source for alpha-galactosidase by Bacillus sp. JF(2
).