USE OF RAPD AND 16S RDNA SEQUENCING FOR THE STUDY OF LACTOBACILLUS POPULATION-DYNAMICS IN NATURAL WHEY CULTURE

Authors
COCCONCELLI PS PARISI MG SENINI L BOTTAZZI V
Citation
Ps. Cocconcelli et al., USE OF RAPD AND 16S RDNA SEQUENCING FOR THE STUDY OF LACTOBACILLUS POPULATION-DYNAMICS IN NATURAL WHEY CULTURE, Letters in applied microbiology, 25(1), 1997, pp. 8-12
Citations number
21
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
25
Issue
1
Year of publication
1997
Pages
8 - 12
Database
ISI
SICI code
0266-8254(1997)25:1<8:UORA1R>2.0.ZU;2-4
Abstract
The development of communities of the thermophilic microflora of natur al whey culture for Parmigiano Reggiano cheese production was studied by means of molecular techniques. RAPD analysis facilitates the identi fication of the Lactobacillus strains involved in this microbial assoc iation and permitted the study of population dynamics during two cycle s of whey fermentation. Analysis of RAPD fingerprints revealed the pre sence of four biotypes that dominate the whey fermentation process. Se quence analysis of 16S rDNA demonstrated that the strains isolated fro m whey belong to Lact. helveticus and Lact. delbrueckii ssp. lactis sp ecies.