THE USE OF SORGHUM FOR THERMOSTABLE AMYLASE PRODUCTION FROM THERMOACTINOMYCES-THALPOPHILUS

Authors
UGURU GC AKINYANJU JA SANI A
Citation
Gc. Uguru et al., THE USE OF SORGHUM FOR THERMOSTABLE AMYLASE PRODUCTION FROM THERMOACTINOMYCES-THALPOPHILUS, Letters in applied microbiology, 25(1), 1997, pp. 13-16
Citations number
16
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
25
Issue
1
Year of publication
1997
Pages
13 - 16
Database
ISI
SICI code
0266-8254(1997)25:1<13:TUOSFT>2.0.ZU;2-C
Abstract
Thermoactinomyces thalpophilus isolated from flour mill wastes was fou nd to produce extracellular amylase in shake flask cultures using sorg hum as carbon source. Sorghum in mineral salts medium significantly su pported a higher rate of amylase synthesis by the organism than solubl e starch, giving peak amylase activity at the stationary phase. The op timum temperature and pH of the enzyme was 90 degrees C and 5.0, respe ctively, with more than 50% enzyme activity retained at 100 degrees C (30 min).