BAROTOLERANCE IS DEPENDENT ON BOTH TREHALOSE AND HEAT-SHOCK-PROTEIN-104 BUT IS ESSENTIALLY DIFFERENT FROM THERMOTOLERANCE IN SACCHAROMYCES-CEREVISIAE

The contribution of trehalose and hsp104 to barotolerance in Saccharom yces cerevisiae has been investigated. Mutant strains, which lacked th e ability to accumulate trehalose and/or hsp104, were examined for bar otolerance and thermotolerance. All the mutants showed lower barotoler ance and thermotolerance than their control strains. Trehalose had a g reater protective effect towards high pressure than high temperature. Thus, trehalose and hsp104 are important factors for barotolerance and thermotolerance, but trehalose is more important for barotolerance th an for thermotolerance.