Mutational analysis of the candidate tumor suppressor gene ING1 in Indian oral squamous cell carcinoma

INGI, a recently identified candidate tumor suppressor gene, involved in th e p53 signaling pathway is mapped at chromosome 13q34. Since loss of hetero zygosity at 13q34 has been reported in squamous cell carcinoma of head and neck, we screened for mutations in INGI by polymerase chain reaction-single strand conformation polymorphism in 71 oral squamous cell carcinomas (OSCC ) from India, 15 of which were known to harbor pn mutations. A single polym orphism (G to A) was detected in 14 (19.7%) of the tumors analyzed. No muta tion was observed in any of the 71 OSCCs analyzed. These results suggest th at INGI is not a target for mutational inactivation in OSCC of Indians. (C) 2001 Elsevier Science Ltd. All rights reserved.