Inhibition of immunologic and nonimmunologic stimulation-mediated anaphylactic reactions by water extract of white eggplant (Solanum melongena)

We investigated the effect of water extract of Solanum melongena (SMWE) on immunologic and nonimmunologic stimulation-mediated anaphylactic reactions. Nonimmunologic anaphylactic reaction was induced by compound 48/80 injecti on. Oral administration of SMWE (I g kg(-1)) completely inhibited compound 48/80-induced anaphylactic reaction. Immunologic anaphylactic reaction was generated by sensitizing the skin with anti-dinitrophenyl (DNP) IgE followe d 48 h later with an injection of antigen, Oral administration of SMWE (0.0 1-1 g kg(-1)) significantly inhibited passive cutaneous anaphylactic reacti on activated by anti-DNP IgE to between 83.10 +/- 1.67% and 70.17 +/- 2.17% . SMWE (0.01-1 mg ml(-1)) also inhibited histamine release activated by com pound 48/80 to between 93 +/- 2.65 and 70 +/- 1.50%. Moreover, SMWE (0.01-1 mg ml(-1)) had a significant inhibitory effect on IgE-induced tumor necros is factor (TNF)-alpha, secretion from rat peritoneal mast cells. These resu lts indicate that SMWE inhibits immunologic and nonimmunologic stimulation- mediated anaphylactic reactions and TNF-alpha secretion from mast cells. (C ) 2001 Academic Press.