A Brassica oleracea gene expressed in a variety-specific manner may encodea novel plant transmembrane receptor

species Brassica oleracea includes several agricultural varieties character ized by the proliferation of different types of meristems, Using a combinat ion of subtractive hybridization and PCR (polymerase chain reaction) techni ques we have identified several genes which are expressed in the reproducti ve meristems of the cauliflower curd (B, oleracea var, botrytis) but not in the vegetative meristems of Brussels sprouts (B, oleracea var, gemmaifera) axillary buds. One of the cloned genes, termed CCE1 (CAULIFLOWER CURD EXPR ESSION 1) shows specific expression in the botrytis variety. preferential e xpression takes place in this variety in the meristems of the curd and in t he stem throughout the vegetative and reproductive stages of plant growth, CCE1 transcripts are not detected in any of the organs of other B, oleracea varieties analyzed. Based on the nucleotide sequence of a cDNA encompassin g the complete coding region, we predict that this gene encodes a transmemb rane protein, with three transmembrane domains. The deduced amino acid sequ ence includes motifs conserved in G-protein-coupled receptors (GPCRs) from yeast and animal species. Our results suggest that the cloned gene encodes a protein belonging to a new, so far unidentified, family of transmembrane receptors in plants. The expression pattern of the gene suggests that the r eceptor may be involved in the control of meristem development/arrest that takes place in cauliflower.