Reversible immobilization of lipase on phenylalanine containing hydrogel membranes

Authors
Arica, MY Kacar, Y Ergene, A Denizli, A
Citation
My. Arica et al., Reversible immobilization of lipase on phenylalanine containing hydrogel membranes, PROCESS BIO, 36(8-9), 2001, pp. 847-854
Citations number
30
Categorie Soggetti
Biotecnology & Applied Microbiology","Biochemistry & Biophysics
Journal title
PROCESS BIOCHEMISTRY
ISSN journal
13595113 → ACNP
Volume
36
Issue
8-9
Year of publication
2001
Pages
847 - 854
Database
ISI
SICI code
1359-5113(200103)36:8-9<847:RIOLOP>2.0.ZU;2-S
Abstract
Poly(2-hydroxyethylmethacrylate-co-methacrylamidophenlyalanine) poly(HEMA-M APA) membranes were prepared by UV-initiated photopolymerization of HEMA an d MAPA. Lipase immobilization onto these membranes from aqueous solutions c ontaining different amounts of lipase at different pH was investigated in a batch system. The lipase adsorption capacity of the membranes was increase d as the MAPA ratio increased in the membrane structure. The maximum lipase immobilization capacity of the poly(HEMA-MAPA-3) membrane was 135 mug cm(- 2). The optimum temperature was 5 degreesC higher than that of the free enz yme and was significantly broader. The storage stability increased with imm obilization. The enzyme could be repeatedly adsorbed and desorbed without a ny significant loss in adsorption capacity. (C) 2001 Elsevier Science Ltd. All rights reserved.