Transcardiac 8-iso-prostaglandin F-2 alpha generation from acute myocardial infarction heart: insight into abrupt reperfusion and oxidant stress

8-iso-prostaglandin F-2 alpha (8-iso-PGF(2 alpha)), a representative isopro stane, has been reported to be a reliable marker for oxidant stress in vivo . To examine if 8-iso-PGF(2 alpha), is generated in patients with acute myo cardial infarction (AMI), we measured the level of immunoreactive 8-iso PGF (2 alpha), in the great cardiac vein as well as classical eicosanoids, 6-ke to-prostaglandin F-1 alpha (6-keto-PGF(1 alpha)) and thromboxane B-2 (TXB2) in the process of urgent coronary balloon angioplasty. Fourteen patients w ith anterior AMI were divided into two groups: the totally occluded (n=7) a nd the already perfused groups (n=7). In the former, transient elevation of 8-iso-PGF(2 alpha), was observed immediately after the angioplasty, i.e. t he ratio of post-angioplasty level to pre-level was approximately 2.4 for 8 -iso-PGF(2 alpha),14 for B-keto-PGF(1 alpha), and 5 forTXB(2). In the alrea dy perfused group, the levels of these eicosanoids were unchanged. In the t otally occluded group, peak creatine phosphokinase in a peripheral vein was correlated with the level of 8-iso-PGF(2 alpha) (r(2)=0.841, P < 0.01), bu t not with those of the other two eicosanoids. In conclusion, transcardiac 8-iso-PGF(2<alpha>), generation is a reliable marker for the size of myocar dium exposed to oxidant stress. (C) 2001 Harcourt Publishers Ltd.