Cytoplasmic illuminations: in planta targeting of fluorescent proteins to cellular organelles

Use of the jellyfish green-fluorescent protein as an in vivo reporter is in the process of revolutionising plant cell biology. By fusing the protein t o specific targeting peptides or to sequences of complete proteins, it is n ow possible to observe the location, structure. and dynamics of a number of intracellular organelles over extended periods of time, In this review we discuss the most recent developments and unexpected results originating fro m the targeting of this unique protein and its derivatives to elements of t he cytoskeleton and to membrane-bounded organelles in a range of plant cell types.