Polymorphisms of the mannose binding lectin gene in patients with Sjogren's syndrome

Objective-To investigate polymorphisms of both codon 54 allele and promoter variants of the mannose binding lectin (MBL) gene in patients with primary Sjogren's syndrome (SS). Methods-Polymorphisms of codon 54 allele and promoter variants of the MBL g ene in 104 patients with SS and 143 healthy controls were determined by pol ymerase chain reaction-restriction fragment length polymorphism and allele specific polymerase chain reaction respectively. Results-The allele frequency of the wild type of MBL codon 54 was significa ntly higher in patients with SS than in controls (0.836 v 0.741; p=0.011), and the frequency of the homozygous wild type of MBL codon 54 was significa ntly higher in patients with SS than in controls (0.692 v 0.539; p=0.024). On the other hand, the allele frequencies of the MBL promoter gene did not differ between patients and controls (chi (2)=4.01, df=2, p=0.135). Conclusion-The polymorphism of the MBL gene may be one of the genetic facto rs that determines susceptibility to SS.