Flow cytometric analysis of gut mucosal lymphocytes supports an impaired Th1 cytokine profile in spondyloarthropathy

Objective-To quantify the fraction of gut mucosal lymphocytes expressing th e T helper type 1 (Th1) cytokines, interferon gamma (IFN gamma) and interle ukin (IL)2, and the Th2 cytokines, IL4 and IL10, at the single cell level i n patients with spondyloarthropathy (SpA) in comparison with healthy contro ls. Methods-An improved extraction protocol was used for the enrichment of intr aepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) from c olonic and ileal biopsy specimens obtained from patients with SpA (n=20) an d healthy controls (n=13). After stimulation with phorbol ester/ionomycin, expression of the intracellular cytokines IFN gamma, IL2, IL4, and IL10 was determined in CD3+, CD3+CD8+ and CD3+CD8- T cells by flow cytometry. Results-In colonic LPLs, a significant decrease in IFN gamma -producing CD3 + cells was observed (p=0.02) in patients with SpA. In the CD3+CD8- subset, the proportion of cells producing IFN gamma and IL2 was decreased in patie nts with SpA (p=0.021 and p=0.027 respectively). In heal LPLs, the percenta ge of IL10-producing CD3+CD8- cells was significantly increased (p=0.046). Conclusion-An impaired Th1 cytokine profile is observed in gut mucosal lymp hocytes from patients with SpA. This adds to the existing evidence that the gut mucosal immune apparatus is involved in the pathogenesis of SpA.