Characterization of three members of murine alpha 1,2-fucosyltransferases:Change in the expression of the Se gene in the intestine of mice after administration of microbes

We cloned three members of a GDP-fucose:beta -galactoside alpha1,2-fucosylt ransferase (alpha1,2-fucosyltransferase) family, MFUT-I, -II, and -III, fro m a cDNA of murine small intestine, and determined their enzymatic properti es after transfection of the genes into COS-7 cells, and their expression i n murine tissues by Northern blotting, MFUT-I, -II, and -III exhibited sequ ence homology with the human H (78.4%), Se (79.0%), and Sec1 (74.9%) gene p roducts, respectively. COS-7 cells transfected with MFUT-I and -II exhibite d alpha1,2-fucosyltransferase activity and the best acceptor substrate for both gene products was GA1 to yield a fucosyl GA1 structure, but no activit y was detected in COS-7 cells with MFUT-III. MFUT-II yielded a 3.5-kb mRNA transcript in several tissues, whereas MFUT-I and -III were predominantly e xpressed in epididymis and testis, respectively. The administration of micr obes into germ-free mice resulted in a rapid increase of the MFUT-II gene ( Se gene) for the synthesis of fucosyl GA1 in the intestine. (C) 2001 Academ ic Press.