Stem cell factor and interleukin-3 induce stepwise generation of erythroidprecursor cells from a basic fibroblast growth factor-dependent hematopoietic stem cell line, A-6

A multipotent; immature myeloid cell population was produced from a basic f ibroblast growth factor (bFGF)-dependent hematopoietic stem cell line, A-6, when cultured with stem cell factor (SCF) replacing bFGF. Those cells were positive for stem cell markers, c-kit and CD34, and a myeloid cell marker, F4/80. Some cell fractions were also positive for Mac-1, a macrophage mark er cap Gr-1, a granulocytic maker, but negative for an erythroid marker TER 119. They also showed the expression of mRNA for the myeloid-specific PU.1 but did not that for the erythroid-specific GATA-1. Among various cytokines , interleukin-3 (IL-3) induced erythroid precursor cells that expressed the erythroid-specific GATA-1 and beta -major globin. The quantitative analysi s showed that erythroid precursor cells mere newly produced from the immatu re myeloid cells by cultivation with IL-3. SCF and IL-3 induced stepwise ge neration of erythroid precursor cells from an A-6 hennatopoietic stem cell line. (C) 2001 Academic Press.