A study of a new TSM bio-mimetic sensor using a molecularly imprinted polymer coating and its application for the determination of nicotine in human serum and urine

Authors
Tan, YG Yin, J Liang, CD Peng, H Nie, LH Yao, SZ
Citation
Yg. Tan et al., A study of a new TSM bio-mimetic sensor using a molecularly imprinted polymer coating and its application for the determination of nicotine in human serum and urine, BIOELECTRO, 53(2), 2001, pp. 141-148
Citations number
34
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOELECTROCHEMISTRY
ISSN journal
03024598 → ACNP
Volume
53
Issue
2
Year of publication
2001
Pages
141 - 148
Database
ISI
SICI code
0302-4598(200103)53:2<141:ASOANT>2.0.ZU;2-P
Abstract
A new bio-mimetic quartz crystal thickness-shear-mode (TSM) sensor, using a n imprinted polymer coating as the sensitive material, has been fabricated and applied to the determination of nicotine (NIC) in human serum and urine . The molecularly imprinted polymer (MIP) was synthesized using NIC as the template molecule and methacrylic acid (MAA) as the functional monomer. The sensor showed high selectivity and a sensitive response to NIC in aqueous system. The linear response range of the sensor was between 5.0 x 10(-8) an d 1.0 x 10(-4) M with a detection limit of 2.5 x 10(-8) M. The viscoelastic ity of the coating in the air and in liquid has been studied by the impedan ce spectrum, The MIP sensor was stable and exhibited effective reproducibil ity. Satisfactory results were achieved in the detection of the real sample s. (C) 2001 Elsevier Science B.V. All rights reserved.