Detection of diagnostically critical, often hidden, anomalies in complex karyotypes of haematological disorders using multicolour fluorescence in situ hybridization

Authors
Jalal, SM Law, ME Stamberg, J Fonseca, R Seely, JR Myers, WH Hanson, CA
Citation
Sm. Jalal et al., Detection of diagnostically critical, often hidden, anomalies in complex karyotypes of haematological disorders using multicolour fluorescence in situ hybridization, BR J HAEM, 112(4), 2001, pp. 975-980
Citations number
11
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
BRITISH JOURNAL OF HAEMATOLOGY
ISSN journal
00071048 → ACNP
Volume
112
Issue
4
Year of publication
2001
Pages
975 - 980
Database
ISI
SICI code
0007-1048(200103)112:4<975:DODCOH>2.0.ZU;2-Q
Abstract
Multicolour fluorescence in situ hybridization (M-FISH) simultaneously dete cts all 24 human chromosomes in unique fluorescent colours. The identificat ion of diagnostically critical gene rearrangement(s) in complex karyotypes of haematological disorders continues to be a challenge. We present five ca ses in which t(9:11), complex t(8:22), t(12;21) and t(11;14) were detected primarily using M-FISH and were confirmed using locus-specific probes. conc lude that M-FISH can be effective in complete characterization of critical gene rearrangements in haematological disorders.