Biological action of nitric oxide donor compounds on platelets from patients with sickle cell disease

Citation
Th. Mondoro et al., Biological action of nitric oxide donor compounds on platelets from patients with sickle cell disease, BR J HAEM, 112(4), 2001, pp. 1048-1054
Citations number
31
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
BRITISH JOURNAL OF HAEMATOLOGY
ISSN journal
00071048 → ACNP
Volume
112
Issue
4
Year of publication
2001
Pages
1048 - 1054
Database
ISI
SICI code
0007-1048(200103)112:4<1048:BAONOD>2.0.ZU;2-K
Abstract
Several lines of evidence point to the potential role of nitric oxide (NO) in the pathophysiology, as well as in the therapy, of sickle cell disease ( SCD). In this study, we compared the effects of NO on platelets from normal individuals and from patients with SCD. Three NO donors were used to deliv er NO to platelets: sodium 2-(N, N-diethylamino)-diazenolate-2-oxide (DEANO ), S-nitrosocysteine (CysNO) and sodium trioxdintrate (OXINO or Angeli's sa lt). ADP-induced platelet aggregation, CD62P expression, PAC-1 binding and calcium elevation were evaluated in paired studies of normal and SCD subjec ts. DEANO significantly reduced aggregation in SCD platelets compared with normal platelets. DEANO similarly reduced the extent of CD62P expression in SCD platelets. All NO donors reduced PAC-1 binding, but there were no sign ificant differences between platelets from normal or SCD subjects. Calcium elevation, as induced by ADP, was not altered by the presence of NO donors. However, when platelets were stimulated with thrombin, there was an increa sed initial response of SCD platelets compared with normal platelets. Taken together, these data suggest that the mode of NO delivery to platelets may produce various physiological responses and the optimization of NO deliver y may contribute to reducing platelet aggregation in sickle cell disease.