Detection of Borrelia burgdorferi DNA in urine of patients with ocular Lyme borreliosis

Aim-To evaluate the diagnostic value of the polymerase chain reaction (PCR) to detect Borrelia burgdorferi DNA in patients with ocular Lyme borreliosi s. Methods-Of 256 consecutive uveitis patients six selected individuals with c linical evidence for Lyme borreliosis and 30 patients with non-lyme uveitis were enrolled. Lyme serology was performed by ELISA and western blotting. Urine samples were examined by an optimised nested polymerase chain reactio n (PCR) protocol. Results-Only four of six uveitis patients suspected for Lyme borreliosis we re ELISA positive, while all six subjects showed a positive western blot. B burgdorferi PCR was positive in all of these six patients. Whereas two of the 30 controls had a positive Lyme serology, B burgdorferi DNA was not det ectable by PCR in any sample from these patients. Conclusions-PCR for the detection of B burgdorferi DNA in urine of uveitis patients is a valuable tool to support the diagnosis of ocular Lyme borreli osis. Moreover, these patients often show a weak humoral immune response wh ich may more sensitively be detected by immunoblotting.