Structure determination of zeta class glutathione transferase from Arabidopsis thaliana

The cis-trans isomerisation of maleylacetoacetate to fumarylacetoacetate oc curs in the latter stage of the tyrosine and phenylalanine catabolic pathwa y. Recently, it has been shown that this maleylacetoacetate isomerase activ ity is performed by the zeta subclass of the glutathione transferase superf amily, which is conserved in a range of species from mammals to plants. The three-dimensional structure of a zeta class glutathione transferase from A rabidopsis thaliana has been served by single isomorphous replacement with anomalous scattering and refined to a final resolution of 1.8 Angstrom. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.