Polymorphism at the glutathione S-transferase: GSTP1 locus is associated with isocyanate-induced asthma: use of an ARMS-PCR assay for the simultaneous determination of (I105-V105) and A(114)-V-114 alleles

Glutathione S-transferase, GSTP1 is an antioxidant enzyme involved in prote ction against oxidative stress. Four GST alleles, GSTP1*A, GSTP1*B, GSTP1*C , and GSTP1*D, derived from polymorphisms at two sites (Ile(105)-Val(105) a nd Ala(114)-Val(114)), have been identified. We have examined polymorphism at both sites in 104 subjects exposed to toluene diisocyanate (TDI) using a n amplification refractory mutation system assay. The data showed significa nt association of GSTP1 Ile(105)-Va(105) polymorphism with TDI-induced asth ma, which is consistent with our earlier findings. Furthermore, we found th at the Ala(114)-Va(114) polymorphism had little additional effect, compared with the Ile(105)-Val(105) polymorphism, on disease risk. (C) 2001 Elsevie r Science Ireland Ltd. All rights reserved.