Detoxification of acrylonitrile by polymorphic human glutathione transferase hGSTP1

Fifty-nine persons exposed to acrylonitrile (AN) were studied. Hemoglobin a dduct monitoring including CEV [N-(cyanoethyl)valine] as biomonitoring para meter for AN exposure was performed. The genotypes of several polymorphic g lutathione transferases and of cytochrome P450 2E1 were determined. The CEV adduct levels correlated significantly with the presence of the mutation a t the GSTP1 A(1404)G locus (w/w < w/m < m/m) but not with the GSTM1, GSTT1 or CYP2E1 genotypes. The CEV levels were also significantly higher in the G STP1 C2294T heterozygotes. According to the presented results AN appears to be metabolized by GSTP1 but not by GSTM1 or GSTT1 in humans. (C) 2001 Else vier Science Ireland Ltd. All rights reserved.