Activation of soluble glutathione transferase in rat hepatoma-derived Fa32cells by several pesticides and oxidants

The influence of chloroacetanilides (alachlor, metolachlor and propachlol), pesticides (carbaryl, quinalphos, benomyl and carbendazim.) and oxidants [ tert-butyl hydroperoxide (BHP), hydrogen peroxide and diamide] on the solub le glutathione transferase (GST) activity and the GST subunit pattern was i nvestigated in rat hepatoma-derived Fa32 cells. No GST activation was obser ved for quinalphos and diamide. The other xenobiotics stimulated the total GST activity to different degrees (111-159% of the control) and BHP to 400% . The GST subunit patterns were analyzed by HPLC. Several alterations as co mpared with control cells were observed, even when the xenobiotics did not activate the total GST activity. We assume that the GST alterations are cor related with a detoxicating effect. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.