Simultaneous analysis of enantiomeric composition of amino acids and N-acetyl-amino acids by enantioselective chromatography

Among the three chiral columns, CHIROBIOTIC T (TM), CHIRLPAK WH, and CHIRAL CEL OD-R, tested for the separation of racemic amino acids and N-acetyl-ami no acids, only CHIROBIOTIC TM chiral column which is based on covalently bo nded amphoteric glycopeptide, teicoplanin, as the stationary phase ligand c ould be successfully developed to enantiomerically separate racemic amino a cids and N-acetyl-amino acids simultaneously. This method can be used to de termine the enantiomeric composition of amino acids and N-acetyl-amino acid s in the catalysis of D-aminoacylase or L-aminoacylase and the conversion r ate of N-acylamino acid racemases. (C) 2001 Wiley-Liss, Inc.