The molecular composition of biophenols in olive fruit was investigated in
order to experiment with novel procedures for the determination of these mi
crocomponents in fresh and processed table olives. The presence of biopheno
ls in table olives, with recognized antioxidant activity, can be strictly l
inked to the texture and the organoleptic characteristics of the food produ
ct, giving a functional value to this Mediterranean food. Olives from Spain
("Hojiblanca" cv.), Portugal ("Douro" cv.), Greece ("Conservolia" and "Tha
sos"cv.) and Italy ("Taggiasca" and "Cassanese" cv.) were examined, because
the experimental data, checked by high-performance liquid chromatography (
HPLC), show molecular composition differences in the tested samples related
to the geographic area of analyzed olive fruit cultivars [Bianco, A., & Uc
cella, N. (2000). Biophenolic components of olives. Food Research Internati
onal, 33, 475-485]. Three different protocols were utilized: the first one
allows the determination of the biophenolic content present as free and est
erified compounds; the second affords the total biophenolic content; the th
ird indicates the biophenols present as glycosides. The biophenolic content
, which was previously determined by classic HPLC methods (Bianco & Uccella
, 2000), was checked by a simple]H-NMR experiment. The comparison between t
he data obtained from H-1-NMR with those measured by HPLC, indicates a good
agreement and suggests the possibility of employing H-1-NMR for the rapid
determination of biophenolic content in olives and also in other foods. (C)
2001 Elsevier Science Ltd. All rights reserved.