S. Bystricky et al., Conjugation of yeast mannans with protein employing cyanopyridinium agent (CDAP) - an effective route of antifungal vaccine preparation, GLYCOCON J, 17(10), 2000, pp. 677-680
The possibility of using 1-cyano-4-dimethylaminopyridinium tetrafluoroborat
e (CDAP) for activation of saccharide hydroxyl groups (instead of hazardous
cyanogen bromide) is examined with cell-surface mannans of the yeasts Cand
ida albicans,Candida tropicalis, Candida lambica and galactoglucoxylomannan
of Cryptococcus laurentii. Direct conjugation with human serum albumin yie
lded soluble products with increased molecular size in comparison with the
original polysaccharides. Immunodiffusion experiments revealed that conjuga
tion did not affect the immunospecificity of the antigen epitope.