Non-steroidal anti-inflammatory drugs with activity against either cyclooxygenase 1 or cyclooxygenase 2 inhibit colorectal cancer in a DMH rodent model by inducing apoptosis and inhibiting cell proliferation
Wa. Brown et al., Non-steroidal anti-inflammatory drugs with activity against either cyclooxygenase 1 or cyclooxygenase 2 inhibit colorectal cancer in a DMH rodent model by inducing apoptosis and inhibiting cell proliferation, GUT, 48(5), 2001, pp. 660-666
Background-Standard non-steroidal anti-inflammatory drugs (NSAIDs) reduce t
he risk of colorectal cancer by 40-60% but the mechanism by which this occu
rs is uncertain. Selective cyclooxygenase2inhibitors arepotentiallyidealche
mopreventive agents as they are less toxic than standard NSAIDs. No study h
as compared the efficacy of these drugs at clinically relevant doses in a t
umour model.
Aims-To assess the efficacy of a range of NSAIDs with varying activity agai
nst the two cyclooxygenase isoforms in a rodent colorectal carcinogen model
at antiinflammatory doses and to explore the effect of NSAIDs on the rate
of tumour apoptosis and proliferation.
Methods-Colorectal tumours were induced in six week old Sprague-Dawley rats
with five weekly doses of 1,2 dimethylhydrazine. Test agents were: indomet
hacin 2 mg/kg/day, meloxicam 0.6 mg/kg/ day, celecoxib 6 mg/kg/day, and sul
indac sulphone 40 mg/kg/day. Sulindac was tested at its chemoprotective dos
e of 20 mg/kg/day. After 23 weeks the number and volume of tumours per anim
al were recorded. Histology was performed. Tumour apoptosis was quantified
on haematoxylin-eosin sections. Tumour proliferation was quantified using a
n immunohistochemical stain for bromodexoyuridine incorporation.
Results-Test agents effectively reduced the number and volume of tumours de
veloping in the treatment period. In all groups there was an increase in th
e rate of tumour apoptosis and a reduced rate of proliferation.
Conclusions-These data suggest that the chemopreventive effect of NSAIDs is
independent of their cyclooxygenase inhibitory profile. One potential mech
anism for their action may be through induction of apoptosis and inhibition
of proliferation.