A novel amplicon at 9p23-24 in squamous cell carcinoma of the esophagus that lies proximal to GASC1 and harbors NFIB

The non-random amplification of DNA at 9p23-24 observed in various types of human cancers, including esophageal squamous cell carcinomas (ESCs), may r eflect the locations of important tumor-associated genes. Our previous stud ies using ESC cell lines defined an amplicon in this region and identified a novel gene, GASC1, as a target of the amplification. Since different regi ons within the same chromosome arm are often involved in amplification in a syntenic or non-syntenic manner, we characterized the amplicon at 9p23-24 in 35 ESC cell lines (29 KYSE series and 6 YES series), and examined possib le involvement of non-syntenic amplifications at 9p23-24 in 32 primary ESCs , Our results clearly indicated that two target regions for DNA amplificati on exist at 9p23-24; the major amplicon contains GASC1, and the minor one h arbors a transcription factor, NFIB, centromeric to the GASC1 locus.